Difference between revisions of "Part:BBa K4806013"

Revision as of 07:16, 13 September 2023

AβSAP(i)-promotor for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (Bba_K4806013) and a terminator like tRPL23 (Bba_K4806008), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (Bba_K4806012) is recommended.

Constructs

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 249
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 530
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 268
1000
COMPATIBLE WITH RFC[1000]

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-<h1>CYP2D6 gene for Chlamydomonas reinhardtii (Phytobrick)</h1>
 <p>This basic part contains the coding sequence of CYP2D6 (B3-B4). This part is codon-optimized for Chlamydomonas reinhardtii and was built as part of the CYPurify Collection. In combination with a promoter like AβSAP(i) (<a href=” https://parts.igem.org/ Bba_K4806013”>Bba_K4806013</a>) and a terminator like tRPL23 (<a href=” https://parts.igem.org/ Bba_K4806008”>Bba_K4806008</a>), this level 0 part leads to expression and potential detoxification of specific chemicals (Ohkawa & Inui, 2015). To detect the target protein a tag like FLAG-tag (<a href=” https://parts.igem.org/ Bba_K4806012”>Bba_K4806012</a>) is recommended. </p>
 <h2><u>Constructs</u></h2>