Difference between revisions of "Part:BBa K194003"

 
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<partinfo>BBa_K194003 short</partinfo>
 
<partinfo>BBa_K194003 short</partinfo>
  
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This biobrick contains a PacI/Nt.BbvCI and a AsiSI/Nb.BtsI USER cassette for insertion of PCR fragments using the USER cloning standard (see http://2009.igem.org/Team:DTU_Denmark/project). PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase.
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This biobrick contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard (see http://2009.igem.org/Team:DTU_Denmark/project). PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 11:18, 16 October 2009

USER cassette for insertion of USER fragments

This biobrick contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard (see http://2009.igem.org/Team:DTU_Denmark/project). PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]