Difference between revisions of "Part:BBa K4656004"
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PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.
 
PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.

Revision as of 15:46, 7 September 2023

PLEE1

PLEE1 is the promoter of the locus of enterocyte effacement which binds to pchA.

Since the specific binding site of pchA and PLEE1 is still unknown, at present, researchers have purchased Escherichia coli genome from atcc and amplified PLEE1 with different fragments by PCR as candidates, and constructed 6 different kinds of plasmids. +1 is the transcription start site TSS.

"Additionally, the best performing plasmid, pC4S-P4, contains a small part of the ler gene in the PLEE1 promoter region."[1] {The pC4S-P4: pDMB,PpchA-pchA-PLEE1(-98+218bp)-gfp;CmR}

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1]. Bai, Y. and T.J. Mansell, Production and Sensing of Butyrate in a Probiotic Escherichia coli Strain. Int J Mol Sci, 2020. 21(10).