Difference between revisions of "Part:BBa K4719020"
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<partinfo>BBa_K4719020 short</partinfo> | <partinfo>BBa_K4719020 short</partinfo> | ||
− | We fused | + | We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain. |
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Revision as of 08:06, 7 September 2023
CBD-ProThr box-ArCE4A chitin deacetylase and cellulose binding domain fusion protein
We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 432
Illegal NgoMIV site found at 961
Illegal AgeI site found at 417 - 1000COMPATIBLE WITH RFC[1000]