Difference between revisions of "Part:BBa K4719017"

Revision as of 08:50, 4 September 2023

phaCAB operon for polyhydroxybutyrate synthesis in K. xylinus

Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331-Bb backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NotI site found at 200
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 642
Illegal BamHI site found at 3039
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 37
Illegal PstI site found at 824
Illegal PstI site found at 1397
Illegal NgoMIV site found at 253
Illegal NgoMIV site found at 368
Illegal NgoMIV site found at 602
Illegal NgoMIV site found at 914
Illegal NgoMIV site found at 1193
Illegal NgoMIV site found at 1606
Illegal NgoMIV site found at 1673
Illegal AgeI site found at 341
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4719017 short</partinfo>
-Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331b backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.
+Polyhydroxybutyrate synthesis operon (phaC, phaA, phaB) assembled into pSEVA331-Bb backbone, to produce PHB along with bacterial cellulose in K. xylinus. This copolymer is an alternative to petroleum-based plastics.
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