Difference between revisions of "Part:BBa K4804000:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Insert PySp1 fragment to the C terminus of PETase using seamless cloning technology.
+
PETase without stop codon
  
  

Latest revision as of 05:57, 25 August 2023


PETase-R


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 871
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 923
    Illegal NgoMIV site found at 965
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PETase without stop codon


Source

PETase from Ideonella sakaiensis. PySp1 from Araneida

References