Difference between revisions of "Part:BBa K4865000:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
There is a polyclonal enzyme cleavage site at the C end of the EGF, where we insert other gene fragment.
+
This encoding sequence does not contain a start codon.
  
  

Latest revision as of 03:21, 25 August 2023


EGF without start condon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 134


Design Notes

This encoding sequence does not contain a start codon.


Source

EGF from Homo sapiens and PelB from Bacillus sp. .

References