Difference between revisions of "Part:BBa K4790042"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4790042 short</partinfo> | <partinfo>BBa_K4790042 short</partinfo> | ||
| − | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 40th amino acid from methionine to glutamic acid. According to Codon preference in E.coli, GAA has the highest possibility coding | + | The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 40th amino acid from methionine to glutamic acid. According to Codon preference in E.coli, GAA has the highest possibility coding glutamic acid. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 07:38, 14 August 2023
ASR1-M40E-F
The primer was designed to perform site-directed mutation which was defined as a forward primer. It can mutate base sequence which is on code 59 and coding the 40th amino acid from methionine to glutamic acid. According to Codon preference in E.coli, GAA has the highest possibility coding glutamic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]