Difference between revisions of "Part:BBa K4765014"
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<partinfo>BBa_K4765008 short</partinfo>
 
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In the final step, nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase MysH
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==Introduction==
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completes the production of palythines from disubstituted MAAs.
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Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>.
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==Usage and Biology==
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We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273014 BBa_K4273014] (NlmysH) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''.
  
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===Usage and Biology===
 
  
 
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==References==
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<references />

Revision as of 07:54, 11 August 2023


INPNC

Introduction

Nonheme iron-(II)- and 2oxoglutarate-dependent (Fe/2OG) oxygenase gene MysH completes the production of palythines from disubstituted MAAs[1].

Usage and Biology

We performed codon optimization on BBa_K4273014 (NlmysH) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysH catalyzes the final reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.