Difference between revisions of "Part:BBa K4765013"
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<partinfo>BBa_K4765007 short</partinfo>
 
<partinfo>BBa_K4765007 short</partinfo>
  
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MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike
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==Introduction==
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enzyme MysD.
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MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>.
  
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<!-- Add more about the biology of this part here
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==Usage and Biology==
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===Usage and Biology===
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We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273013 BBa_K4273013] (NlmysD) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''.
  
 
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<partinfo>BBa_K4765007 parameters</partinfo>
 
<partinfo>BBa_K4765007 parameters</partinfo>
 
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==References==
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<references />

Revision as of 07:52, 11 August 2023


Nb3, R4P43 from 10.1016/j.cell.2018.06.041

Introduction

MAA analogues like shinorine or porphyra-334 is transformed from MG by a D-Ala-D-Ala ligaselike enzyme gene MysD[1].

Usage and Biology

We performed codon optimization on BBa_K4273013 (NlmysD) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysD catalyzes the fourth reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.