Difference between revisions of "Part:BBa K4765012"
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| − | An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine | + | ==Introduction== |
| − | (MG). | + | An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine (MG)<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>. |
| − | + | ==Usage and Biology== | |
| − | + | We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273008 BBa_K4273008] (NpR5598) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysC catalyzes the third reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''. | |
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<partinfo>BBa_K4765006 parameters</partinfo> | <partinfo>BBa_K4765006 parameters</partinfo> | ||
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| + | ==References== | ||
| + | <references /> | ||
Revision as of 07:50, 11 August 2023
Ag3, P53TA from 10.1016/j.cell.2018.06.041
Introduction
An ATP-grasp enzyme MysC introduces L-Glycine to 4-DG at C3, and produces mycosporineglysine (MG)[1].
Usage and Biology
We performed codon optimization on BBa_K4273008 (NpR5598) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysC catalyzes the third reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.