Difference between revisions of "Part:BBa K4765011"

 
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MysB (O-MT), an O-methyltrans-ferase, converts Demethyl 4-deoxygadusol into 4-deoxygadusol
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==Introduction==
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(4-DG), completing the production of 4-DG, the basic substrate of MAAs.
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MysB (O-MT), an O-methyltrans-ferase, converts Demethyl 4-deoxygadusol into 4-deoxygadusol (4-DG), completing the production of 4-DG, the basic substrate of MAAs<ref>Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. ''The Journal of Organic Chemistry, 86''(16), 11160–11168.</ref>.
  
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==Usage and Biology==
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===Usage and Biology===
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We performed codon optimization on [https://parts.igem.org/Part:BBa_K4273005 BBa_K4273005] (NpR5599) specifically for the ''Escherichia coli'' K12 strain, resulting in the creation of this part. The enzyme MysB catalyzes the second reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within ''E. coli''.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K4765005 parameters</partinfo>
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<partinfo>BBa_K4765011 parameters</partinfo>
 
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==References==
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<references />

Revision as of 07:48, 11 August 2023


MysB codon optimized

Introduction

MysB (O-MT), an O-methyltrans-ferase, converts Demethyl 4-deoxygadusol into 4-deoxygadusol (4-DG), completing the production of 4-DG, the basic substrate of MAAs[1].

Usage and Biology

We performed codon optimization on BBa_K4273005 (NpR5599) specifically for the Escherichia coli K12 strain, resulting in the creation of this part. The enzyme MysB catalyzes the second reaction involved in the biosynthesis of Mycosporine-like amino acids (MAAs) within E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 532


References

  1. ↑ Chen, M., Rubin, G. M., Jiang, G., Raad, Z., & Ding, Y. (2021). Biosynthesis and heterologous production of mycosporine-like amino acid palythines. The Journal of Organic Chemistry, 86(16), 11160–11168.