Difference between revisions of "Part:BBa K4765015"
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AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. *Gene*, *539*(1), 132–140.https://doi.org/10.1016/j.gene.2014.01.013</ref>. We heterologously expressed codon-optimized AnAFP in *Escherichia coli*, endowing it with antifreeze capability. | AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions<ref>Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. *Gene*, *539*(1), 132–140.https://doi.org/10.1016/j.gene.2014.01.013</ref>. We heterologously expressed codon-optimized AnAFP in *Escherichia coli*, endowing it with antifreeze capability. | ||
Revision as of 08:01, 5 August 2023
image demonstration
AnAFP is an antifreeze protein derived from *Ammopiptanthus nanus*. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1]. We heterologously expressed codon-optimized AnAFP in *Escherichia coli*, endowing it with antifreeze capability.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
- ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. *Gene*, *539*(1), 132–140.https://doi.org/10.1016/j.gene.2014.01.013