Difference between revisions of "Part:BBa K4727000"

 
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This backbone has been made standard RFC[10] compatible and provided with the RFC[10] prefix and suffix. Flanked by prefix and suffix is located part <partinfo>BBa_J23119</partinfo> that has been demonstrated able to promote mRNA synthesis in <i>K. pneumoniae</i> and <i>A. baumannii</i>
 
This backbone has been made standard RFC[10] compatible and provided with the RFC[10] prefix and suffix. Flanked by prefix and suffix is located part <partinfo>BBa_J23119</partinfo> that has been demonstrated able to promote mRNA synthesis in <i>K. pneumoniae</i> and <i>A. baumannii</i>
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The plasmid brings AmpR as a selection marker
  
 
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<span class='h3bb'>Sequence and Features</span>
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==Sequence and Features==
 
<partinfo>BBa_K4727000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4727000 SequenceAndFeatures</partinfo>
  

Revision as of 14:41, 25 July 2023


A. baumannii & K. pneumoniae standard backbone

This plasmid backbone was developed by Wang et al [1], it is provided of two ORIs: the first one a pMB1/pUC high copy number ORI for E. coli, the second one is capable o maintaining the plasmid both in Acinetobacter baumannii and Klebsiella pneumoniae

This backbone has been made standard RFC[10] compatible and provided with the RFC[10] prefix and suffix. Flanked by prefix and suffix is located part BBa_J23119 that has been demonstrated able to promote mRNA synthesis in K. pneumoniae and A. baumannii

The plasmid brings AmpR as a selection marker

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 3664
    Illegal suffix found at 3721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3664
    Illegal NheI site found at 969
    Illegal NheI site found at 3692
    Illegal NheI site found at 3715
    Illegal SpeI site found at 3722
    Illegal PstI site found at 3736
    Illegal NotI site found at 3670
    Illegal NotI site found at 3729
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3664
    Illegal BglII site found at 1162
    Illegal XhoI site found at 1775
    Illegal XhoI site found at 3780
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3664
    Illegal suffix found at 3722
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3664
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3679
    Illegal SpeI site found at 3722
    Illegal PstI site found at 3736
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 4008