Difference between revisions of "Part:BBa K4727004"

Latest revision as of 20:45, 24 July 2023

M13 Gene3 N2 deletion

The design of this part was based on the fundamental work of DA Marvin[1], which suggested that mutants lacking the N2 domain og Gp3 protein of M13 bacteriphage can infect bacteria without pilus with a 100-fold higher efficiency[1], as N2 masks the interaction of N1 with the co-receptor TolA. Building on this, the idea was to design a gene with a deleted N2 domain, drawing from the findings of Nilsson et al.[2], who precisely delineated the amino acid regions corresponding to individual domains. Therefore, an alternative gene was designed for tropism that could ideally enable E. coli infection in the absence of a pilus, thus extending the tropism. This gene, named was obtained by in silico deletion of the region between residues 87 and 256, corresponding to the N2 domain[2].

Sequence and Features

References

[1] Marvin, D. Filamentous phage structure, infection and assembly. Curr. Opin. Struct. Biol. 8, 150–158 (1998). [2] Nilsson, N., Malmborg, A.-C. & Borrebaeck, C. A. K. The Phage Infection Process: a Functional Role for the Distal Linker Region of Bacteriophage Protein 3. J. Virol. 74, 4229–4235 (2000)