Difference between revisions of "Part:BBa J433115"

 
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Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin from Asimov Mammalian Collection
 
Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin from Asimov Mammalian Collection
 
Clone in with BsaI GCTT and TACA Fusion Sites
 
Clone in with BsaI GCTT and TACA Fusion Sites
−
Clone out into L2 with SapI AGG and ATA Fusion Sites
+
Clone out into L2 with SapI AGG and ACC Fusion Sites
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:13, 26 June 2023


Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin

Type IIS L1 TU1 Cloning Plasmid SpecR ColE1 v3 origin from Asimov Mammalian Collection Clone in with BsaI GCTT and TACA Fusion Sites Clone out into L2 with SapI AGG and ACC Fusion Sites

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 1921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 1776
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2050
    Illegal SapI.rc site found at 5