Difference between revisions of "Part:BBa J435116"

 
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Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.
 
Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.
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https://freegenes.github.io/genes/BBF10K_000493.html
  
 
One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148
 
One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Latest revision as of 16:24, 23 May 2023


pOSIP-TT (TetR, P21)

pOSIP-TT (TetR, P21)

One-Step Cloning and Chromosomal Integration of DNA

Contains ccdB, so must be grown in a strain resistant to this product (e.g. DB3.1). Growth a 30C to repress expression of the integrase.

https://freegenes.github.io/genes/BBF10K_000493.html

One-step cloning and chromosomal integration of DNA. St-Pierre F, Cui L, Priest DG, Endy D, Dodd IB, Shearwin KE. ACS Synth Biol. 2013 Sep 20;2(9):537-41. doi: 10.1021/sb400021j. Epub 2013 May 20. 10.1021/sb400021j PubMed 24050148

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1125
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 5490
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 6090
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal NotI site found at 1133
    Illegal NotI site found at 5025
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BglII site found at 469
    Illegal BamHI site found at 22
    Illegal XhoI site found at 1152
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1126
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 5490
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 28
    Illegal XbaI site found at 1397
    Illegal XbaI site found at 5490
    Illegal SpeI site found at 1126
    Illegal PstI site found at 1140
    Illegal AgeI site found at 1343
    Illegal AgeI site found at 3052
    Illegal AgeI site found at 4681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 357
    Illegal BsaI.rc site found at 3197