Difference between revisions of "Part:BBa J435400"

Latest revision as of 10:01, 10 May 2023

T4T5_Int_ThiolS

Modified intein, cleavable by addition of thiol-containing molecules such as cysteine, DTT, and beta-mercaptoethanol. Defined as a T4T5 part.

Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.

FLANKED 5' fusion site: TTCG, 3' fusion site: CGGC

Source organism: N/A, Target organism: Escherichia coli

Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003461.html

Sequence and Features