Difference between revisions of "Part:BBa J435393"

 
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A TEV protease recognition domain, HIS tag, and stop codon. Defined as a DF part.
 
A TEV protease recognition domain, HIS tag, and stop codon. Defined as a DF part.
  
−
FLANKED 5' fusion site: AGGT, 3' fusion site: GCTT
+
'''Note:''' Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.
 +
 
 +
FLANKED 5' fusion site: AGGT, 3' fusion site: CGCT
  
 
Source organism: N/A, Target organism: Escherichia coli
 
Source organism: N/A, Target organism: Escherichia coli

Latest revision as of 09:56, 10 May 2023


DF_TEVcut_His_Stop_TZ

A TEV protease recognition domain, HIS tag, and stop codon. Defined as a DF part.

Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.

FLANKED 5' fusion site: AGGT, 3' fusion site: CGCT

Source organism: N/A, Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003459.html

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NotI site found at 218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal BamHI site found at 70
    Illegal XhoI site found at 227
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]