Difference between revisions of "Part:BBa J435392"

 
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T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.
 
T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.
  
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FLANKED 5' fusion site: A, 3' fusion site: AGGT
+
'''Note:''' Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.
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 +
FLANKED 5' fusion site: A, 3' fusion site: GCTT
  
 
Source organism: , Target organism: Escherichia coli
 
Source organism: , Target organism: Escherichia coli

Latest revision as of 09:52, 10 May 2023


CE_T7RNApol_Stop

T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.

Note: Possible misidentification. Flanking sites on FreeGenes appear to be wrong, possibly incorrect BBF10K number identified.


FLANKED 5' fusion site: A, 3' fusion site: GCTT

Source organism: , Target organism: Escherichia coli


Citation: https://docs.google.com/spreadsheets/d/1uUS6TaIy0YuT8W7hdeonu0NSpAHinieyPcvgZRG1Nos/edit#gid=1530011293

ORG: E. coli Protein Expression Toolkit, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_003441.html

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2592
    Illegal AgeI site found at 1804
  • 1000
    COMPATIBLE WITH RFC[1000]