Difference between revisions of "Part:BBa K3443002"

Latest revision as of 03:55, 14 October 2022

PthlA constitutive promotor from C. acetobutylicum

PthlA is a constitutive promotor fund in Clostridium acetobutylicum. It can be used in artificial acetone synthesis plasmids. Here it regulates the expression of genes: thlA, ctfA/ctfB and adc.

Improvement from NJTch_China 2022

Document

Results:

The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.

Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 ===Usage and Biology===
-<!-- -->
-<span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K3443002 SequenceAndFeatures</partinfo>
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 <partinfo>BBa_K3443002 parameters</partinfo>
 <!-- -->
+===Improvement from NJTch_China 2022===
+<html lang="en">
+<head>
+    <meta charset="UTF-8">
+    <meta http-equiv="X-UA-Compatible" content="IE=edge">
+    <meta name="viewport" content="width=device-width, initial-scale=1.0">
+    <title>Document</title>
+</head>
+<body>
+    <p style="font-size: 180%; font-weight: bold;">Results:</p>
+    <p>The fluorescence intensity shows that the promoter thlF7 has a trends in aerobic inhibition. But the performence in microaerobic situation is also inhibited.</p>
+    <p><img src="https://static.igem.wiki/teams/4119/wiki/jt-files/h2/img12.png" width="80%" height="80%"></p>
+    <div align="center">
+        <strong>Fig.2. Expression effect of PthlA and PthlAF7-bs2 at different oxygen concentrations</strong>
+    </div>
+</body>
+</html>
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K3443002 SequenceAndFeatures</partinfo>