Difference between revisions of "Part:BBa K4429012:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | FcRn binding peptide variant generated according to Sockolosky et al 2012 [1], attached at the right location on the scFv BBa_K4429007 using SnapGene. Protein sequence reverse translated and optimised for expression in E. coli K12. | ||
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===References=== | ===References=== | ||
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+ | [1] Sockolosky, J. T., Tiffany, M. R., & Szoka, F. C. (2012). Engineering neonatal Fc receptor-mediated recycling and transcytosis in recombinant proteins by short terminal peptide extensions. Proceedings of the National Academy of Sciences of the United States of America, 109(40), 16095–16100. https://doi.org/10.1073/pnas.1208857109 |
Latest revision as of 03:54, 14 October 2022
NeoFv513 VH-LinY12H-VL
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
FcRn binding peptide variant generated according to Sockolosky et al 2012 [1], attached at the right location on the scFv BBa_K4429007 using SnapGene. Protein sequence reverse translated and optimised for expression in E. coli K12.
Source
-
References
[1] Sockolosky, J. T., Tiffany, M. R., & Szoka, F. C. (2012). Engineering neonatal Fc receptor-mediated recycling and transcytosis in recombinant proteins by short terminal peptide extensions. Proceedings of the National Academy of Sciences of the United States of America, 109(40), 16095–16100. https://doi.org/10.1073/pnas.1208857109