Difference between revisions of "Part:BBa K4414001"
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==Usage and Biology== | ==Usage and Biology== | ||
| − | A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus(Cokol et al., 2000) . We obtained this DNA by DNA synthesis | + | A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus(Cokol et al., 2000) . We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory. |
Latest revision as of 03:45, 14 October 2022
nuclear localization signal（NLS）
This nuclear localization sequence（NLS） derived from SV40 directs the protein into the nucleus.
Usage and Biology
A nuclear localization signal (NLS) is a short stretch of amino acids that mediates the transport of nuclear proteins to the nucleus. The NLS motif plays a key role in this mechanism; (i) normally, deletion of NLS disrupts nuclear import; (ii) if fused to NLS, normally non-nuclear proteins are imported into the nucleus(Cokol et al., 2000) . We obtained this DNA by DNA synthesis.This part can be used in level 1 biological laboratory.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
1 .Cokol, M., Nair, R., & Rost, B. (2000). Finding nuclear localization signals. EMBO Reports, 1(5), 411–415. https://doi.org/10.1093/embo-reports/kvd092