Difference between revisions of "Part:BBa K4429013"

 
 
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NeoFvs are engineered antibody fragments. An scFv is defined as the variable domains of a full length antibody connected via a flexible linker. A NeoFv is a name given by iGEM 2022 team IISER-Pune2_India to scFvs with a small peptide extension that binds to the human neonatal Fc receptor (or FcRn receptor). Such scFvs with an FcRn binding peptide have recently been described in literature [1] with potential to overcome the size problem of scFvs, and extend their half life of circulation in the human body.
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NeoFv513 VH-VL-LinY12H is one such NeoFv derived from the Ab513 antibody targeting the envelope protein domain III epitope of the Dengue virus. A NeoFv derived from Ab513 can ideally be a therapy for Dengue fever.
  
 
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<partinfo>BBa_K4429013 parameters</partinfo>
 
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===References===
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*1]Short FcRn-Binding Peptides Enable Salvage and Transcytosis of scFv Antibody Fragments
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Vince W. Kelly and Shannon J. Sirk
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ACS Chemical Biology 2022 17 (2), 404-413
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DOI: 10.1021/acschembio.1c00862

Latest revision as of 03:36, 14 October 2022


NeoFv513 VH-VL-LinY12H

NeoFvs are engineered antibody fragments. An scFv is defined as the variable domains of a full length antibody connected via a flexible linker. A NeoFv is a name given by iGEM 2022 team IISER-Pune2_India to scFvs with a small peptide extension that binds to the human neonatal Fc receptor (or FcRn receptor). Such scFvs with an FcRn binding peptide have recently been described in literature [1] with potential to overcome the size problem of scFvs, and extend their half life of circulation in the human body.

NeoFv513 VH-VL-LinY12H is one such NeoFv derived from the Ab513 antibody targeting the envelope protein domain III epitope of the Dengue virus. A NeoFv derived from Ab513 can ideally be a therapy for Dengue fever.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

  • 1]Short FcRn-Binding Peptides Enable Salvage and Transcytosis of scFv Antibody Fragments

Vince W. Kelly and Shannon J. Sirk ACS Chemical Biology 2022 17 (2), 404-413 DOI: 10.1021/acschembio.1c00862