Difference between revisions of "Part:BBa K4227026:Design"

(→‎Design Notes)
(→‎References)
 
Line 15: Line 15:
  
 
===References===
 
===References===
−
[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Springer Science and Business Media LLC.2013;1 (64): 31-38.
+
[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology.2013;1 (64) : 31-38.
  
−
[2] Lutz, R., and Bujard, H. (1997) Independent and Tight Regulation of Transcriptional Units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 Regulatory Elements. Nucleic Acids Res.25 (6), 1203−1210
+
[2] R Lutz. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research.1997;6 (25) : 1203-1210.
  
−
[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. American Chemical Society (ACS).2020;10 (15) : 2833-2841. Sequence and Features
+
[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. ACS Chemical Biology.2020;10 (15) : 2833-2841.<p> Sequence and Features

Latest revision as of 03:30, 14 October 2022


PnlmC-xip-P34-sigma28


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After an engineering cycle, we failed in verification the muB. And the modeling shows that the express level of the muB is much lower than other proteins.

In order to have higher level of the expression, we learned the literature and choose the xip-P34,a kind of short peptide which only contains 9 aa.

Source

Synthesized by the Tsingke Biotechnology Co., Ltd.

References

[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology.2013;1 (64) : 31-38.

[2] R Lutz. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research.1997;6 (25) : 1203-1210.

[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. ACS Chemical Biology.2020;10 (15) : 2833-2841.

Sequence and Features