Difference between revisions of "Part:BBa K4227026"
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===Characterization===
 
===Characterization===
 
<p><strong> (The following experimental data are from the literature and modeling, not the actual data made by the experiment) </strong></p>
 
<p><strong> (The following experimental data are from the literature and modeling, not the actual data made by the experiment) </strong></p>
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<p>Since our experiment is severely influenced by COVID-19 in experimenter, materials, equipment and so on this year. We have to collect these data by the literature and modeling</p>
 
<p>Since our experiment is severely influenced by COVID-19 in experimenter, materials, equipment and so on this year. We have to collect these data by the literature and modeling</p>
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<p>Our modeling was based on the law of mass action.</p>
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<div style="display: flex;justify-content:center;align-items:center;flex-direction: column;">
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<div>
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<img src="https://static.igem.org/mediawiki/parts/9/9c/Xxxiiippp.png">
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<p style="text-align: center;">Fig 1. The expression level of the XIP-P34</p>
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<p>We </p>
 
  
  

Revision as of 03:27, 14 October 2022


PnlmC-xip-P34-sigma28

Usage and Biology

The CSP is the component stimulating peptides of the S.mutans. When there is CSP be sensed by the comE, the comE will be phosphorylated and start the PnlmC promoter, and the muB will be expressed to decompose the EPS[1]

σs have been divided into four groups based on their phylogenetic relationships and modular structure. σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flagella-related genes in all motile Gram-negative and Gram-positive bacteria and plays a role in development in some non-motile bacteria. We use the σ28 to start the P28 promotor[2]

The xip-P34 is a kind of analogue of the XIP signal peptide. It can be a kind of competitive inhibitors of comX promotor, so we use this to inhibit the EPS foundation[3]

Characterization

(The following experimental data are from the literature and modeling, not the actual data made by the experiment)

Since our experiment is severely influenced by COVID-19 in experimenter, materials, equipment and so on this year. We have to collect these data by the literature and modeling

Our modeling was based on the law of mass action.

<img src="Xxxiiippp.png">

Fig 1. The expression level of the XIP-P34





Reference

[1] Tianlei Liu, Shoubin Xue, Wenbo Cai, Xiaojing Liu, Xiaojuan Liu, Rongrong Zheng, Hongyan Luo, Wenhui Qi. ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Springer Science and Business Media LLC.2013;1 (64): 31-38.

[2] Lutz, R., and Bujard, H. (1997) Independent and Tight Regulation of Transcriptional Units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-1-I-2 Regulatory Elements. Nucleic Acids Res.25 (6), 1203−1210

[3] Chowdhury Raihan Bikash, Yftah Tal-Gan. Structure Activity Relationship Study of the XIP Quorum Sensing Pheromone in Streptococcus mutans Reveal Inhibitors of the Competence Regulon. American Chemical Society (ACS).2020;10 (15) : 2833-2841. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]