Difference between revisions of "Part:BBa K4297042"

Latest revision as of 03:10, 14 October 2022

PYLXP'-PAR1

The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde. The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 762.71 mg/L.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1293
Illegal NheI site found at 5520
Illegal NotI site found at 1448
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 249
Illegal XhoI site found at 4316
Illegal XhoI site found at 4349
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4638
Illegal NgoMIV site found at 4668
Illegal NgoMIV site found at 4901
Illegal NgoMIV site found at 5133
Illegal NgoMIV site found at 5304
Illegal AgeI site found at 950
Illegal AgeI site found at 4585
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI site found at 1284
Illegal BsaI site found at 4588
Illegal BsaI.rc site found at 255
Illegal BsaI.rc site found at 4582
Illegal BsaI.rc site found at 4767
Illegal BsaI.rc site found at 5621
Illegal SapI.rc site found at 5042

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4297042 short</partinfo>
-The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE.
+The plasmid pYLXP' contains the expressiom cassette of alcohol dehydrogenase PAR1, which can catalyze the reaction of phenylacetaldehyde to 2-PE. Next, we transformed plasmids into Y. lipolytica and perform the whole-cell conversion of phenylacetaldehyde.  The whole-cell biocatalytic conversion of 1 g/L phenylacetaldehyde by aforementioned strains gave 2-PE titers of 762.71 mg/L.
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