Difference between revisions of "Part:BBa K4438108:Experience"
 
Line 6: Line 6:
 
===Applications of BBa_K4438108===
 
===Applications of BBa_K4438108===
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146_(30_min).png]]
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146_(30_min).png]]
−
The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
+
 
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146-(1_hr).png]]
 
[[File:T--IISER-Tirupati_India--fluroscence_miR-146-(1_hr).png]]
−
The fluorescence readout shown above observes a spike in the highest concentration of dye and miDNA. This supports the fact that the formation of G-quadruplex is facilitated by the addition of the biomarker.
+
 
 +
Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA
 +
 
 +
Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
 +
 
 +
 
 +
While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K4438108 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K4438108 StartReviews</partinfo>

Latest revision as of 03:06, 14 October 2022


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K4438108

T--IISER-Tirupati India--fluroscence miR-146 (30 min).png

T--IISER-Tirupati India--fluroscence miR-146-(1 hr).png

Depicting the fluorescence readout of FASTmiR-146a-D4 (design 4) after 30 mins and 1 hour incubation respectively. Control 1: DFHBI dye + buffer; Control 2: DFHBI dye + buffer + FASTmiR; Exp: DFHBI dye + buffer + FASTmiR + miDNA

Explanation: After 30 minutes of incubation with 5X sensor buffer and dye, there was a dramatic shift in fluorescence intensity when the FASTmiR-146a sensor was increased in concentration. An average fold-change of 1.327 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.319 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.


While after 1 hour of incubation with 5X sensor buffer and dye, an average fold-change of 1.41 was observed after the addition of miDNA-146a throughout a range of DNA concentrations, with a maximum fold-change of 3.311 observed at a DNA concentration of 1.8 μM and a FASTmiR-146a concentration of 90 nM.

User Reviews

UNIQ303ea34bae82bfdb-partinfo-00000000-QINU UNIQ303ea34bae82bfdb-partinfo-00000001-QINU