Difference between revisions of "Part:BBa K4297059"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4297059 short</partinfo>
 
<partinfo>BBa_K4297059 short</partinfo>
  
−
plasmid pYLXP' containing the expressiom cassette of the recombinase Cre
+
Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I-like mechanism to carry out site specific recombination events.
 +
 
  
  

Latest revision as of 02:57, 14 October 2022


pYLXP

Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I-like mechanism to carry out site specific recombination events.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1293
    Illegal NotI site found at 1448
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 249
    Illegal XhoI site found at 4316
    Illegal XhoI site found at 4349
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5930
    Illegal NgoMIV site found at 5960
    Illegal AgeI site found at 950
    Illegal AgeI site found at 4585
    Illegal AgeI site found at 5013
    Illegal AgeI site found at 6067
    Illegal AgeI site found at 6186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI site found at 1284
    Illegal BsaI site found at 4588
    Illegal BsaI site found at 5016
    Illegal BsaI site found at 6070
    Illegal BsaI.rc site found at 255
    Illegal BsaI.rc site found at 4582
    Illegal BsaI.rc site found at 4600
    Illegal BsaI.rc site found at 5010
    Illegal BsaI.rc site found at 6064
    Illegal BsaI.rc site found at 6505