Difference between revisions of "Part:BBa K4429000:Design"
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===References=== | ===References=== | ||
[1] https://www.imgt.org/ligmdb/view?id=X07174 | [1] https://www.imgt.org/ligmdb/view?id=X07174 | ||
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[2] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072 | [2] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072 | ||
Latest revision as of 02:34, 14 October 2022
Anti-MBP Recombinant IgY Light Chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ensuring the correct V-C region interface for murine and chicken antibody, removing BioBrick Illegal restriction sites
Source
Anti-MBP Variable Light region found from the genetic sequence of anti-MBP IgG expressed in SHuffle E. coli [2]. The constant light region was found in the germline sequence of chickens (through the IMGT database [1]). The assembled coding sequence was codon optimised for E. coli K12.
References
[1] https://www.imgt.org/ligmdb/view?id=X07174
[2] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072