Difference between revisions of "Part:BBa K4429000:Design"
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===Source=== | ===Source=== | ||
| − | Anti-MBP Variable Light region found from the genetic sequence of anti-MBP IgG expressed in SHuffle E. coli. The constant light region was found in the germline sequence of chickens (through the IMGT database). The assembled coding sequence was codon optimised for E. coli K12. | + | Anti-MBP Variable Light region found from the genetic sequence of anti-MBP IgG expressed in SHuffle E. coli. The constant light region was found in the germline sequence of chickens (through the IMGT database [1]). The assembled coding sequence was codon optimised for E. coli K12. |
===References=== | ===References=== | ||
Revision as of 02:33, 14 October 2022
Anti-MBP Recombinant IgY Light Chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Ensuring the correct V-C region interface for murine and chicken antibody, removing BioBrick Illegal restriction sites
Source
Anti-MBP Variable Light region found from the genetic sequence of anti-MBP IgG expressed in SHuffle E. coli. The constant light region was found in the germline sequence of chickens (through the IMGT database [1]). The assembled coding sequence was codon optimised for E. coli K12.