Difference between revisions of "Part:BBa K4429001:Design"
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[1] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072 | [1] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072 | ||
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| + | [2] https://www.imgt.org/ligmdb/view?id=X07174 | ||
Latest revision as of 02:32, 14 October 2022
Anti-MBP Recombinant IgY Heavy Chain
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 870
Illegal SapI.rc site found at 1450
Design Notes
Assembled from Variable Heavy regions against Maltose Binding Protein, and Gallus gallus IgY constant region taken from IMGT [2].
Consideration was taken to ensure the correct V-C region interface between a mouse IgG variable region (aMBP) and Chicken IgY constant region.
Codon optimisation performed for E. coli K12, removing biobrick illegal restriction sites. Adding polyhistidene tag.
Source
anti-MBP variable region sequence comes from the coding sequence of anti-MBP IgG expressed in SHuffle E. coli in this Robinson et al. 2015 [1].
References
[1] Robinson, MP., Ke, N., Lobstein, J. et al. Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria. Nat Commun 6, 8072 (2015). https://doi.org/10.1038/ncomms9072