Difference between revisions of "Part:BBa K4429001:Design"
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| − | Codon optimisation for E. coli K12, removing restriction sites | + | Codon optimisation for E. coli K12, removing biobrick illegal restriction sites. Adding polyhistidene tag. |
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===Source=== | ===Source=== | ||
Revision as of 02:27, 14 October 2022
Anti-MBP Recombinant IgY Heavy Chain
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 870
Illegal SapI.rc site found at 1450
Design Notes
Codon optimisation for E. coli K12, removing biobrick illegal restriction sites. Adding polyhistidene tag.
Source
anti-MBP variable region sequence comes from the coding sequence of anti-MBP IgG expressed in SHuffle E. coli in this Robinson et al. 2015 [1].