Difference between revisions of "Part:BBa K4438201"

Latest revision as of 01:19, 14 October 2022

Spinach 2.3

Spinach 2.3 is a mutant version of Spinach 2.1(BBa_K1330000), made to increase the fluorescent intensity given out by the aptamer on binding with DFHBI.

Usage and Biology

The free energy of the thermodynamic ensemble for the sequence below is predicted to be -34.44 kcal/mol. The tetraloop is highlighted in red text, and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence, and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 ===Usage and Biology===
 The free energy of the thermodynamic ensemble for the sequence below is predicted to be -34.44 kcal/mol. The tetraloop is highlighted in red text, and the inversions are underlined. The sequences highlighted in bold are the aptamer sequence, and those flanking are the tRNA scaffold sequence, tRNALys3. The mutation and inversions are indicated in the RNAfold structures using blue circles.
+[[File:Parts-mod-Figure1_(1).jpg]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K4438201 SequenceAndFeatures</partinfo>
-[[File:Parts-mod-Figure1_(1).jpg]]