Difference between revisions of "Part:BBa K4238003"
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===Wet lab result===
 
===Wet lab result===
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with using BBa_K4238004 as a reporter, we tested how EL222 activates C120-trcuCYC1 promoter under the blue light.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:53, 14 October 2022


Act1p+NLS-VP16-EL222+CYC1t
This part is responsible for the acceptance of blue light in yeast cells. EL222 dimerizes under blue light and binds specifically to a particular DNA sequence (C120). NUS Singapore 2022 created Basic Parts by adding the transcription inducers VP16 and NLS to EL222 to promote specific expressions under blue light. We have implemented this part (BBa_K3570021) with the Constitutive promoter ACT1 and the CYC1 terminator in yeast.

Wet lab result

with using BBa_K4238004 as a reporter, we tested how EL222 activates C120-trcuCYC1 promoter under the blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 801
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 801
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 801
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 801
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 801
  • 1000
    COMPATIBLE WITH RFC[1000]