Difference between revisions of "Part:BBa K4475006:Design"

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Genome of Cercospora Beticola
 
Genome of Cercospora Beticola
 
https://www.ncbi.nlm.nih.gov/gene/35424646
 
https://www.ncbi.nlm.nih.gov/gene/35424646
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Taken From
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BBa_K4475000:Design
  
 
===References===
 
===References===
  
 
Newman AG, Townsend CA. Molecular Characterization of the Cercosporin Biosynthetic Pathway in the Fungal Plant Pathogen Cercospora nicotianae. J Am Chem Soc. 2016 Mar 30;138(12):4219-28. doi: 10.1021/jacs.6b00633. Epub 2016 Mar 16. PMID: 26938470; PMCID: PMC5129747.
 
Newman AG, Townsend CA. Molecular Characterization of the Cercosporin Biosynthetic Pathway in the Fungal Plant Pathogen Cercospora nicotianae. J Am Chem Soc. 2016 Mar 30;138(12):4219-28. doi: 10.1021/jacs.6b00633. Epub 2016 Mar 16. PMID: 26938470; PMCID: PMC5129747.

Revision as of 21:46, 13 October 2022


CTB3 Gene from Cercospora beticola


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1263
    Illegal BglII site found at 388
    Illegal BglII site found at 562
    Illegal BglII site found at 1291
    Illegal BglII site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1263
    Illegal XbaI site found at 100
    Illegal XbaI site found at 457
    Illegal XbaI site found at 925
    Illegal XbaI site found at 1243
    Illegal XbaI site found at 1381
    Illegal XbaI site found at 1651
    Illegal XbaI site found at 2104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cut site eligibility for RFC 1000 achieved through silent mutations. N-terminal 6xHis tagged and codon optimized for yeast.

Source

Genome of Cercospora Beticola https://www.ncbi.nlm.nih.gov/gene/35424646

Taken From

BBa_K4475000:Design

References

Newman AG, Townsend CA. Molecular Characterization of the Cercosporin Biosynthetic Pathway in the Fungal Plant Pathogen Cercospora nicotianae. J Am Chem Soc. 2016 Mar 30;138(12):4219-28. doi: 10.1021/jacs.6b00633. Epub 2016 Mar 16. PMID: 26938470; PMCID: PMC5129747.