Difference between revisions of "Part:BBa K4212028"
 
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Destination plasmid containing a sfGFP dropout.
 
Destination plasmid containing a sfGFP dropout.
  
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===Usage and Biology===
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[[File:b28.jpg|600px|thumb|center|Figure 1. The schematic graphic of Level1 plasmid for 1B construct containing a sfGFP dropout cassette.]]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 19:38, 13 October 2022


STK-1B-sfGFP

Destination plasmid containing a sfGFP dropout.

Figure 1. The schematic graphic of Level1 plasmid for 1B construct containing a sfGFP dropout cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
    Illegal PstI site found at 2130
    Illegal NotI site found at 1058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1052
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal PstI site found at 2130
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1052
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1067
    Illegal PstI site found at 2130
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2108
    Illegal BsaI.rc site found at 1090
    Illegal SapI.rc site found at 1274