Difference between revisions of "Part:BBa K4212027"

 
 
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Destination plasmid containing a sfGFP dropout
 
Destination plasmid containing a sfGFP dropout
  
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===Usage and Biology===
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[[File:b27.jpg|600px|thumb|center|Figure 1. The schematic graphic of Level1 plasmid for 1A construct containing a sfGFP dropout cassette.]]
  
 
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Latest revision as of 19:35, 13 October 2022


STK-1A-sfGFP

Destination plasmid containing a sfGFP dropout

Figure 1. The schematic graphic of Level1 plasmid for 1A construct containing a sfGFP dropout cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
    Illegal XbaI site found at 1043
    Illegal PstI site found at 2106
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
    Illegal PstI site found at 2106
    Illegal NotI site found at 1034
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1028
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1028
    Plasmid lacks a suffix.
    Illegal PstI site found at 2106
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1028
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1043
    Illegal PstI site found at 2106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2084
    Illegal BsaI.rc site found at 1066
    Illegal SapI.rc site found at 1250