Difference between revisions of "Part:BBa K4212064"

 
 
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Entry-level plasmid containing a sfGFP dropout cassette.
 
Entry-level plasmid containing a sfGFP dropout cassette.
  
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===Usage and Biology===
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[[File:b64.jpg|600px|thumb|center|Figure 1. The schematic graphic of Level0 plasmid containing a sfGFP dropout cassette.]]
  
 
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Latest revision as of 19:27, 13 October 2022


STK-0-sfGFP

Entry-level plasmid containing a sfGFP dropout cassette.


Figure 1. The schematic graphic of Level0 plasmid containing a sfGFP dropout cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal XbaI site found at 465
    Illegal PstI site found at 1507
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal PstI site found at 1507
    Illegal NotI site found at 456
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 450
    Illegal XhoI site found at 326
    Illegal XhoI site found at 2524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 450
    Plasmid lacks a suffix.
    Illegal PstI site found at 1507
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 450
    Plasmid lacks a suffix.
    Illegal XbaI site found at 465
    Illegal PstI site found at 1507
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 661