Difference between revisions of "Part:BBa K4438602"
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<partinfo>BBa_K4438602 short</partinfo> | <partinfo>BBa_K4438602 short</partinfo> | ||
− | T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part | + | T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | This part along with | + | This part along with T6_Trigger_1_midtrigger_phi29 (<partinfo>BBa_K4438601</partinfo>) and Aptamer_T6 (<partinfo>BBa_K4438600</partinfo>) can be used for sensing different concentrations of testosterone. |
[[File:T--IISER-Tirupati_India--T6_1.png]] | [[File:T--IISER-Tirupati_India--T6_1.png]] | ||
Revision as of 18:39, 13 October 2022
T6_Target_1
T6_Target_1 is a single-stranded DNA sequence having 100 nucleotides. Figure 1C) shows the secondary structure and minimum free energy. Its 5’ end has complementary nucleotides for part T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) to bind. The middle region has an ssDNA sense T7 promoter sequence and the 3’ end has an ssDNA sense broccoli light-up aptamer sequence.
Usage and Biology
This part along with T6_Trigger_1_midtrigger_phi29 (BBa_K4438601) and Aptamer_T6 (BBa_K4438600) can be used for sensing different concentrations of testosterone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Skouridou, V., Jauset-Rubio, M., Ballester, P., Bashammakh, A. S., El-Shahawi, M. S., Alyoubi, A. O., & O’Sullivan, C. K. (2017). Selection and characterization of DNA aptamers against the steroid testosterone. Microchimica Acta, 184(6), 1631-1639.