Difference between revisions of "Part:BBa K4323002"

Latest revision as of 18:14, 13 October 2022

Truncated FitD

This part is a truncated version of FitD, a molluscicide from Pseudomonas protegens. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in Escherichia coli. This part is a different form of FitD in comparison to BBa_K2683006. The composite part BBa_K4323003 includes the necessary components to express truncated FitD toxin. For more information, please visit our Wiki page.

Figure 1. The toxicity of Truncated FitD to Dreissena bugensis.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 909
Illegal BamHI site found at 1019
Illegal BamHI site found at 2568
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2191
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4323002 short</partinfo>
-This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in ,i.Escherichia coli,/i.. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].  The composite part [https://parts.igem.org/Part:BBa_K4323003 BBa_K4323003] includes the necessary components to express truncated FitD toxin.  For more information, please visit our [https://2022.igem.wiki/umanitoba/ Wiki page].
+This part is a truncated version of FitD, a molluscicide from <em>Pseudomonas protegens</em>. It is a toxin that can be used to kill quagga and zebra mussels. This part is a coding sequence with a polyhistidine tag for purification. This part is codon optimized for expression in <i>Escherichia coli</i>. This part is a different form of FitD in comparison to [https://parts.igem.org/Part:BBa_K2683006 BBa_K2683006].  The composite part [https://parts.igem.org/Part:BBa_K4323003 BBa_K4323003] includes the necessary components to express truncated FitD toxin.  For more information, please visit our [https://2022.igem.wiki/umanitoba/ Wiki page].
 [[File:FitD toxicity.png|800px|center|FitD toxicity]]