Difference between revisions of "Part:BBa K4391002"
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4391002 short</partinfo>
 
<partinfo>BBa_K4391002 short</partinfo>
  
−
This is an aptamer against Salmonella.
+
This is an high affinity ssDNA aptamer against bacteria <i>Salmonella typhumurium</i> . The aptamer 3D folded structure was generated, molecular docking against most of the known surface proteins of <i>S. typhimurium</i> was performed to identify the protein(s) the aptamer may bind to. Then the molecular dynamics simulation was performed.
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
−
This is an aptamer against Salmonella.
+
 
  
  
Line 14: Line 14:
 
===Modelling===
 
===Modelling===
  
−
===Characterization===
 
−
Boo Baa
 
  
 
===References===
 
===References===
−
[1]
+
[1] Duan, Nuo & Wu, Shijia & Chen, Xiujuan & Huang, Yukun & Xia, Yu & Ma, Xiaoyuan & Wang, Zhouping. (2013). Selection and Characterization of Aptamers against Salmonella typhimurium Using Whole-Bacterium Systemic Evolution of Ligands by Exponential Enrichment (SELEX). Journal of agricultural and food chemistry. 61. 10.1021/jf400767d.
 
<br>
 
<br>
−
[2]
+
[2] http://hex.loria.fr/
 
<br>
 
<br>
−
[3]
+
[3] Zhao, Y., et al., Automated and fast building of three-dimensional RNA structures. Scientific Reports, 2012. 2: p. 734.
 
<br>
 
<br>
−
[4]
+
[4]  
 
<br>
 
<br>
 
[5]
 
[5]

Revision as of 18:01, 13 October 2022

Salmonella typhimurium Aptamer

This is an high affinity ssDNA aptamer against bacteria Salmonella typhumurium . The aptamer 3D folded structure was generated, molecular docking against most of the known surface proteins of S. typhimurium was performed to identify the protein(s) the aptamer may bind to. Then the molecular dynamics simulation was performed.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Modelling

References

[1] Duan, Nuo & Wu, Shijia & Chen, Xiujuan & Huang, Yukun & Xia, Yu & Ma, Xiaoyuan & Wang, Zhouping. (2013). Selection and Characterization of Aptamers against Salmonella typhimurium Using Whole-Bacterium Systemic Evolution of Ligands by Exponential Enrichment (SELEX). Journal of agricultural and food chemistry. 61. 10.1021/jf400767d.
[2] http://hex.loria.fr/
[3] Zhao, Y., et al., Automated and fast building of three-dimensional RNA structures. Scientific Reports, 2012. 2: p. 734.
[4]
[5]