Difference between revisions of "Part:BBa K4307041"

Latest revision as of 17:10, 13 October 2022

OR2-OR3

There are two operators in the genome of bacteriophage, called OR (the right operator) and OL (the left operator) respectively. OR plays an important role in the regulation of gene expression of phage. OR composed of three parts, called OR1, OR2 and OR3. The isolated OR2-OR3 can function as promoter and can be inhibited by Cro protein, a derepressor expressed by bacteriophage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Characterization

The following figure demonstrates our successful construction.

Figure 1: The construction results of OR2-OR3.

@@ Line 12: / Line 12: @@
 <partinfo>BBa_K4307041 SequenceAndFeatures</partinfo>
+<html>
+<h2>Characterization</h2>
+<p>The following figure demonstrates our successful construction.</p>
+<br>
+<div class="center">
+    <div class="thumb tnone">
+        <div class="thumbinner" style="width:50%;">
+            <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307041-gel.png" class="image">
+                <img alt="" src="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307041-gel.png" width="100%" height=auto class="thumbimage" /></a>                  <div class="thumbcaption">
+                <div class="magnify">
+                    <a href="https://static.igem.wiki/teams/4307/wiki/wiki-parts-files/mainpage/bba-k4307041-gel.png" class="internal" title="Enlarge"></a>
+                </div>
+                <b>Figure 1: </b> <b>The construction results of OR2-OR3.</b>
+            </div>
+        </div>
+    </div>
+</div>
+<br>
+</html>
 <!-- Uncomment this to enable Functional Parameter display