Difference between revisions of "Part:BBa K4245202:Design"

Revision as of 16:43, 13 October 2022

hsa-miR-122-5p RCA Padlock Probe
A padlock probe, which is often 30-150 nucleotides in length, is a single-stranded DNA sequence designed to recognize a specific target sequence. The “arms” of a padlock probe are the ends of the ssDNA that are complementary to a specific target sequence. The middle sequence (the sequence between the arms) can be specifically designed to perform a function once amplified. (Nilsson et al., 1994)

For miRNA 122-5p, we designed two complementary arms, BBa_K4245101, the 3' arm for hsa-miR-122-5p and BBa_K4245108, the 5' arm for hsa-miR-122-5p. For the reporter, we decided on both BBa_K4245130/ BBa_K4245132 the FAM and BHQ1 labeled linear probes, and BBa_K4245134/ BBa_K4245135, the DNA fluorescent aptamer split lettuce, due to their frugality and similar wavelength to GFP.

References:

Nilsson, M., Malmgren, H., Samiotaki, M., Kwiatkowski, M., Chowdhary, B. P., & Landegren, U. (1994). Padlock probes: Circularizing oligonucleotides for localized DNA detection. Science, 265(5181), 2085–2088. https://doi.org/10.1126/science.7522346

Design Notes

Source

hsa-miR-122-5p RCA Padlock Probe

References