Difference between revisions of "Part:BBa K4307012"
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Originated from <i>Lactococcus lactis subsp. Lactis</i>, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in <i>E.coli</i> for post-translational modification of NisA-affi fusion protein.<br> | Originated from <i>Lactococcus lactis subsp. Lactis</i>, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in <i>E.coli</i> for post-translational modification of NisA-affi fusion protein.<br> | ||
− | Functioned together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC ( | + | Functioned together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC (BBa_K4307046) to produce functional NisA-affibody peptide. |
Revision as of 16:33, 13 October 2022
NisC
Originated from Lactococcus lactis subsp. Lactis, NisC is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisC in E.coli for post-translational modification of NisA-affi fusion protein.
Functioned together with NisA-affi(BBa_K4307010) and NisB(BBa_K4307011), it forms composite part NisA-affi-NisB+NisC (BBa_K4307046) to produce functional NisA-affibody peptide.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 541
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 541
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 541
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 541
- 1000COMPATIBLE WITH RFC[1000]
Characterization
The following figure demonstrates our successful construction.