Difference between revisions of "Part:BBa K4307011"

Revision as of 16:32, 13 October 2022

NisB

Originated from Lactococcus lactis subsp. Lactis, NisB is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisB in E.coli for post-translational modification of NisA-affi fusion protein.
Functioned together with NisA-affi(BBa_K4307010) and NisC(BBa_K4307012), it forms composite part NisA-affi-NisB+NisC (BBa_K4307046) to produce functional NisA-affibody peptide.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 679
Illegal PstI site found at 2845
Illegal PstI site found at 2944
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 679
Illegal PstI site found at 2845
Illegal PstI site found at 2944
Illegal NotI site found at 1385
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 687
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 679
Illegal PstI site found at 2845
Illegal PstI site found at 2944
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 679
Illegal PstI site found at 2845
Illegal PstI site found at 2944
1000
COMPATIBLE WITH RFC[1000]

Characterization

The following figure demonstrates our successful construction.

Figure 1: The construction results of NisB.

Revision as of 11:25, 13 October 2022 (view source) Liucong20 (Talk \| contribs) m ← Older edit		Revision as of 16:32, 13 October 2022 (view source) Oldluckyzhang (Talk \| contribs) m Newer edit →
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	Originated from <i>Lactococcus lactis subsp. Lactis</i>, NisB is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisB in <i>E.coli</i> for post-translational modification of NisA-affi fusion protein.<br>		Originated from <i>Lactococcus lactis subsp. Lactis</i>, NisB is involved in the post-translational modification of the lantibiotic nisin. We managed to express NisB in <i>E.coli</i> for post-translational modification of NisA-affi fusion protein.<br>
−	Functioned together with NisA-affi(BBa_K4307010) and NisC(BBa_K4307012), it forms composite part NisA-affi-NisB+NisC (~~BBa_K4307014~~) to produce functional NisA-affibody peptide.	+	Functioned together with NisA-affi(BBa_K4307010) and NisC(BBa_K4307012), it forms composite part NisA-affi-NisB+NisC (BBa_K4307046) to produce functional NisA-affibody peptide.