Difference between revisions of "Part:BBa K4444017:Design"

(→‎Design Notes)
 
Line 1: Line 1:
 +
<html lang="en">
 +
<head>
 +
    <meta charset="UTF-8">
 +
    <title></title>
 +
    <style>
 +
        .ui.medium.image {
 +
            width: 100%;
 +
        }
 +
    </style>
 +
    <script type="text/javascript">
 +
        $(this).find('ref').each(function () {
 +
            var label = $(this).text();
 +
            $(this).click(function (event) {
 +
                event.preventDefault();
 +
            });
 +
            var number = figures[1][label];
 +
            $(this).html("<a href=''>Figure " + figures[1][label] + "</a>");
 +
            $(this).on('click', function () {
 +
                window.scroll(0, $(figures[0][number]).offset().top + 100);
 +
            })
 +
        });
 +
        function enumerateKeys(object) {
 +
            var numberPairs = {};
 +
            var objectPairs = {};
 +
            var number = 0;
 +
            var matches = document.body.querySelectorAll(object + '[data-ref]');
 +
            while (number < matches.length) {
 +
                var refName = matches[number].dataset.ref;
 +
                if (refName in numberPairs) {
 +
                    console.log(refName, "This label has already been assigned.");
 +
                } else {
 +
                    numberPairs[number + 1] = matches[number];
 +
                    objectPairs[refName] = number + 1;
 +
                }
 +
                number++;
 +
            }
 +
            return [numberPairs, objectPairs, number];
 +
        }
 +
        function enumerate() {
 +
            var figures = enumerateKeys('figure');
 +
            var maximum = figures[2];
 +
            for (var number = 1; number <= maximum; number++) {
 +
                if (number in figures[0]) {
 +
                    $(figures[0][number]).children('figcaption').prepend("Figure " + number + ": ")
 +
                }
 +
            }
 +
            $(this).find('ref').each(function () {
 +
                var label = $(this).text();
 +
                $(this).click(function (event) {
 +
                    event.preventDefault();
 +
                });
 +
                var number = figures[1][label];
 +
                $(this).html("<a href=''>Figure " + figures[1][label] + "</a>");
 +
                $(this).on('click', function () {
 +
                    window.scroll(0, $(figures[0][number]).offset().top - 110);
 +
                })
 +
            });
 +
        }
 +
        $(document).ready(enumerate);
 +
    </script>
 +
</head>
 +
<body>
 +
</html>
 +
 +
 +
 
===Design Notes===
 
===Design Notes===
  
−
<u>Modifications to FRB-nTEV:
+
<u>Modifications to FRB-nTEV:</u> (<html><ref>1</ref></html>)
  
−
</u>- FRB is replaced with iLID
+
- FRB is replaced with iLID
  
 
- ER localization tag is added at beginning
 
- ER localization tag is added at beginning
Line 10: Line 76:
  
  
−
<u>Components:
+
<u>Components:</u>
 +
 
 +
<html>
 +
<div>
 +
    <figure data-ref="1">
 +
        <img class="nTEViLID" src="https://static.igem.wiki/teams/4444/wiki/parts-pictures/ilidntev.png" width="500">
 +
        <figcaption>The nTEV-iLID System contains ER localization tag, iLID, mCherry, nTEV
  
−
</u>ER localization tag, iLID, mCherry, nTEV
+
        </figcaption>
 +
    </figure>
 +
</div>
 +
</html>
  
−
https://static.igem.wiki/teams/4444/wiki/parts-pictures/ilidntev.png
 
  
 
===Source===
 
===Source===

Latest revision as of 16:02, 13 October 2022


Design Notes

Modifications to FRB-nTEV: (1)

- FRB is replaced with iLID

- ER localization tag is added at beginning

- mCherry is added between iLID and nTEV


Components:

The nTEV-iLID System contains ER localization tag, iLID, mCherry, nTEV


Source

FKBP-nTEV Backbone: https://www.addgene.org/179675/

mCherry: https://www.addgene.org/164037/

iLID: Lab member’s plasmid (William Benman) – pHR sspB(nano)-BFP-SOS-2A-iLID

References

[1] Wehr, M., Laage, R., Bolz, U. et al. Monitoring regulated protein-protein interactions using split TEV. Nat Methods 3, 985–993 (2006). https://doi.org/10.1038/nmeth967

[2] Vlahos, Alexander E., et al. “Protease-Controlled Secretion and Display of Intercellular Signals.” Nature Communications, vol. 13, no. 1, 17 Feb. 2022, p. 912, www.nature.com/articles/s41467-022-28623-y, 10.1038/s41467-022-28623-y.