Difference between revisions of "Part:BBa K4390058"
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<partinfo>BBa_K4390058 short</partinfo>
 
<partinfo>BBa_K4390058 short</partinfo>
  
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
  
 
==Usage and Biology==
 
==Usage and Biology==

Revision as of 15:45, 13 October 2022


His-tagged sfGFP expression construct (JUMP assembled)

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

Usage and Biology

This part contains sfGFP fused with 6x-his tag. This part is used as control for his-tagged MT transformation and expressions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 128