Difference between revisions of "Part:BBa K4165178"
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Amyloid 𝛽 seed (37-42) which has the ability to bind to A𝛽 plaques inside the brain. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis. | Amyloid 𝛽 seed (37-42) which has the ability to bind to A𝛽 plaques inside the brain. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis. | ||
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Latest revision as of 13:49, 13 October 2022
Biobrick GST - Seed
This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), GST tag (BBa_K4165070), Seed peptide (BBa_K4165012), and T7 terminator (BBa_K731721). The GST tag was attached to the Seed peptide coding sequence to serve in the purification using Glutathione resin column.
Usage and Biology
Amyloid 𝛽 seed (37-42) which has the ability to bind to A𝛽 plaques inside the brain. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 763
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 173
Dry lab
Mathematical modeling
Transcription rate and translation rate under T7 promotor
the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.
Figure 1. this figure shows the results from the transcription and translation code showing the
variation of mRNA and protein concentrations with time.