Difference between revisions of "Part:BBa K4497027"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K4497027 short</partinfo>
 
<partinfo>BBa_K4497027 short</partinfo>
  
−
ACTAGTTCTAGAGCGGCCGCTGCAGGAATTCCGGGCCGCGGAGGCTGGATCGGTCCCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTAAACGAGCTCTGCTTATATAGGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGTTTACCACTCCCTATCAGTGATAGAGAAAAGTGAAAGTCGAGCTCGGTACCCGGGTCGAGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT
+
The Bidirectional TRE-CMVmin Promoter a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate the amount of a protein of interest.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 17: Line 17:
 
<partinfo>BBa_K4497027 parameters</partinfo>
 
<partinfo>BBa_K4497027 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===References===
 +
Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g

Latest revision as of 13:11, 13 October 2022


Bidirectional TRE-CMVmin Promoter

The Bidirectional TRE-CMVmin Promoter a bidirectional, Tet-inducible expression vector that allows you to reversibly regulate the amount of a protein of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
    Illegal NotI site found at 476
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
    Illegal XbaI site found at 484
    Illegal SpeI site found at 490
    Illegal PstI site found at 471
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Daringer, N. M., Dudek, R. M., Schwarz, K. A., & Leonard, J. N. (2014). Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices. https://doi.org/10.1021/sb400128g